Niche-specific macrophage loss promotes skin capillary aging

3D imaging of skin macrophage populations. Macrophages - Csf1r-EGFP (green); Collagen - Second Harmonics (blue).

3D imaging of skin tissue niches. All cells - Rosa26-mTomato (red); Collagen - Second Harmonics (blue); Red Blood Cells - Third Harmonics (white).

Time-lapse recording of capillary blood flow. Macrophages - Csf1r-EGFP (green); Red Blood Cells - Third Harmonics (white); Intravenous Rhodamine Dextran (red).

Skin capillary-associated macrophages (CAMs). CAMs - Cx3cr1-GFP (green); All cells - Rosa26-mTomato (red); Collagen - Second Harmonics (blue); Red Blood Cells - Third Harmonics (white).

Time-lapse recording of inconsistent and obstructed blood flow in capillary segments without macrophages. CAMs - Cx3cr1-CreER;Rosa26-mGFP (green); All cells - Rosa26-mTomato (red); Red Blood Cells - Third Harmonics (white).

Time-lapse recording following laser-induced capillary clot formation with rapid migration of CAMs toward site of clot. CAMs - Cx3cr1-CreER;Rosa26-mGFP (green); All cells - Rosa26-mTomato (red); Red Blood Cells - Third Harmonics (white).